Asticcacaulis tiandongensis sp. nov., a new member of the genus Asticcacaulis , isolated from a cave soil sample

A Gram-stain negative, aerobic, motile and rod-shaped bacterium, designated strain 3.1105 , was isolated from a karst district soil sample collected from Tiandong cave, Guizhou province, south-west PR China. The isolate grew at 10-40 °C and pH 5.0-8.0 and tolerated up to 1 % NaCl (w/v) on R2A medium, with optimal growth at 25-30 °C, pH 7.0 and 0 % NaCl (w/v). Cells showed oxidase-positive and catalase-positive reactions. The respiratory quinone was Q-10. The predominant cellular fatty acids contained C ω7 11-methyl, summed feature 8 (C ω7 or C ω6 ), C and C . The major polar lipids were phosphatidylglycerol and monoglycosyldiglycerides. The genomic DNA G+C content was 56.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that 3.1105 should be affiliated to the genus and showed highest 16S rRNA gene sequence similarity values with CB 48 (96.0 %), ZFGT-14 (95.3 %) and lower than 95.3 % similarity to other species of the genus . The polyphasic taxonomic characteristics indicated that strain 3.1105 represents a novel species of the genus , for which the name sp. nov., (type strain 3.1105 =KCTC 62978 =CCTCC AB 2018268 ) is proposed.