Geomonas soli gen. nov., sp. nov., a New Member of the Family Comamonadaceae, Isolated from Soil

A Gram-stain negative, facultative aerobic bacterial strain, designated strain S-16 T , was isolated from soil in South Korea. Colonies were white-milkish and cells were non-motile rods with oxidase- and catalase-positive activities. The growth of strain S-16 T was observed at 20–40 °C (optimum, 25–30 °C) and pH 5.5–7.0 (optimum, pH 6.5). Ubiquinone-8 was identified as the sole respiratory quinone and C 12:0 , C 16:0 , C 18:0 , C 15:1 ω 5 c and summed feature 3 (comprising C 16:1 ω 7 c and/or C 16:1 ω 6 c ) were identified as the major fatty acids (>5%). The major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified polar lipid. The G + C content of the genomic DNA calculated from the whole genome sequence was 66.8 mol%. Strain S-16 T was most closely related to Piscinibacter aquaticus IMCC1728 T , Rhizobacter gummiphilus NS21 T and Rhizobacter dauci H6 T with 16S rRNA gene sequence similarities of 97.93%, 97.93% and 97.44%, respectively. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences suggested that strain S-16 T could form a distinct phyletic lineage as a new genus within the family Comamonadaceae . Based on the phenotypic, chemotaxonomic and molecular features, strain S-16 T represents the type strain of a novel species of a novel genus within the family Comamonadaceae , for which the name Geomonas soli gen. nov., sp. nov. is proposed. The type strain is S-16 T (= KACC 19792 T = JCM 32971 T ).