Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv. Muzao

•A galacturonic acid-rich polysaccharide (PZMP3-2) was purified from jujube fruit.•Structural characteristics of PZMP3-2 was analyzed by GPC, UV, FT-IR, GC-MS, NMR.•Structural features of PZMP3-2 was characterized by SEM, XRD, AFM. Jujube (Zizyphus jujuba Mill.) have been widely used as a health foo...

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Veröffentlicht in:International journal of biological macromolecules 2020-03, Vol.147, p.844-852
Hauptverfasser: Ji, Xiaolong, Yan, Yizhe, Hou, Chunyan, Shi, Miaomiao, Liu, Yanqi
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Sprache:eng
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Zusammenfassung:•A galacturonic acid-rich polysaccharide (PZMP3-2) was purified from jujube fruit.•Structural characteristics of PZMP3-2 was analyzed by GPC, UV, FT-IR, GC-MS, NMR.•Structural features of PZMP3-2 was characterized by SEM, XRD, AFM. Jujube (Zizyphus jujuba Mill.) have been widely used as a health food and medicinal herb in oriental medicine. In the present study, a novel galacturonic acid-rich polysaccharide (PZMP3-2) was isolated from Zizyphus Jujuba cv. Muzao through hot water extraction, ethanol precipitation, and purification with DEAE-Sepharose Fast Flow and Sephacryl S-300 column chromatography. The chemical structures of PZMP3-2 were elucidated by methylation analysis, along with HPGPC, FT-IR spectroscopy, GC-MS, 1D NMR spectroscopy, and 2D NMR spectroscopy. Its morphological properties were further characterized by SEM, AFM, and XRD. Monosaccharide compositional analysis of PZMP3-2 revealed the presence of rhamnose, arabinose, galactose, and galacturonic acid at a molar ratio of 1.74:2.00: 1.00:18.69, and the HPGPC data demonstrated an average molecular weight of 58.21 kDa. Structural and linkage analysis by GC-MS and NMR spectroscopy suggested that PZMP3-2 was a homogalacturonan (HG)-like pectic polysaccharide branched at C-2 with some Araf and Rhap residues. Such unique structure of PZMP3-2 may indicate distinct bioactivities and further application in food and even clinic.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.09.244