Cryo-EM structure of the spinach cytochrome b6 f complex at 3.6 Å resolution

The cytochrome b 6 f (cyt b 6 f ) complex has a central role in oxygenic photosynthesis, linking electron transfer between photosystems I and II and converting solar energy into a transmembrane proton gradient for ATP synthesis 1 – 3 . Electron transfer within cyt b 6 f occurs via the quinol (Q) cycle, which catalyses the oxidation of plastoquinol (PQH 2 ) and the reduction of both plastocyanin (PC) and plastoquinone (PQ) at two separate sites via electron bifurcation 2 . In higher plants, cyt b 6 f also acts as a redox-sensing hub, pivotal to the regulation of light harvesting and cyclic electron transfer that protect against metabolic and environmental stresses 3 . Here we present a 3.6 Å resolution cryo-electron microscopy (cryo-EM) structure of the dimeric cyt b 6 f complex from spinach, which reveals the structural basis for operation of the Q cycle and its redox-sensing function. The complex contains up to three natively bound PQ molecules. The first, PQ1, is located in one cyt b 6 f monomer near the PQ oxidation site (Q p ) adjacent to haem b p and chlorophyll a . Two conformations of the chlorophyll a phytyl tail were resolved, one that prevents access to the Q p site and another that permits it, supporting a gating function for the chlorophyll a involved in redox sensing. PQ2 straddles the intermonomer cavity, partially obstructing the PQ reduction site (Q n ) on the PQ1 side and committing the electron transfer network to turnover at the occupied Q n site in the neighbouring monomer. A conformational switch involving the haem c n propionate promotes two-electron, two-proton reduction at the Q n site and avoids formation of the reactive intermediate semiquinone. The location of a tentatively assigned third PQ molecule is consistent with a transition between the Q p and Q n sites in opposite monomers during the Q cycle. The spinach cyt b 6 f structure therefore provides new insights into how the complex fulfils its catalytic and regulatory roles in photosynthesis. A 3.6 Å resolution cryo-electron microscopy structure of the dimeric cytochrome b 6 f complex from spinach reveals the structural basis for operation of the quinol cycle and its redox-sensing function.