An electrochemical signal-on apta-cyto-sensor for quantitation of circulating human MDA-MB-231 breast cancer cells by transduction of electro-deposited non-spherical nanoparticles of gold
•Non-spherical nanoparticles of gold were electrodeposited.•A single aptamer was immobilized on the nanoparticles of gold.•An electrochemical apta-cyto-sensor for the MDA-MB-231 cells was developed.•The apta-cyto-sensor was label-free, and signal-on. A highly simple, sensitive, specific and low-cost...
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Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2020-01, Vol.178, p.112948-112948, Article 112948 |
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Sprache: | eng |
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Zusammenfassung: | •Non-spherical nanoparticles of gold were electrodeposited.•A single aptamer was immobilized on the nanoparticles of gold.•An electrochemical apta-cyto-sensor for the MDA-MB-231 cells was developed.•The apta-cyto-sensor was label-free, and signal-on.
A highly simple, sensitive, specific and low-cost electrochemical apta-cyto-sensor for determination of circulating human MDA-MB-231 breast cancer cells was fabricated. Non-spherical nanoparticles of gold were electro-deposited in the presence of ethosuximide as a shape directing and size controlling agent. The nanoparticles had dimensions ranging 50–150 nm, and covered the underlying surface with a roughness factor of 8.03. The Non-spherical nanoparticles were then employed as the apta-cyto-sensor transducer. A 83-mer DNA aptamer that is specific to capturing the cell surface proteins was immobilized on the transducer surface, and binding with the cells was followed using the ferro/ferricyanide redox marker. The aptamer was immobilized within ∼200 min on the transducer surface. The cells were quantified with an equation of regression of ΔIp(μA) = (1.028 ± 0.027) log (C (cell mL−1)) + (0.2199 ± 0.0944), a sensitivity of 1.028 μA (log (concentration / cell mL−1))−1 and a quantitation limit of 2 cell mL−1, in a concentration range of 5 to 2 × 106 cell mL−1. The apta-cyto-sensor selectivity was also evaluated toward AsPC-1, Calu-6, HeLa, MCF-7 and melanoma B16/F10 cell lines. The apta-cyto-sensor had a fabrication reproducibility of 4.2%, regeneration capability of 5.1%, a stability of 35 days, and a potential application for the detection of MDA-MB-231 cells in the spiked blood serum samples with a sensitivity of 0.8975 μA (log (concentration / cell mL−1))−1 and a quantitation limit of 5 cell mL−1, in a concentration range of 10 to 1 × 103 cell mL−1. The apta-cyto-sensor would be applicable for breast cancer diagnosis at early stage. |
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ISSN: | 0731-7085 1873-264X |
DOI: | 10.1016/j.jpba.2019.112948 |