Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/β-catenin signaling pathways

Probiotics could be considered as attractive candidates for preventing tumor growth through maintaining homeostasis. The aim of this study was to evaluate the inhibitory effect of a cocktail of five Lactobacillus species on human colorectal carcinoma cell line HT-29. The anti-proliferative and apopt...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Microbial pathogenesis 2020-02, Vol.139, p.103829-103829, Article 103829
Hauptverfasser:	Ghanavati, Roya, Asadollahi, Parisa, Shapourabadi, Mina Bahrololomi, Razavi, Shabnam, Talebi, Malihe, Rohani, Mahdi
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-proliferation Apoptosis beta Catenin - genetics beta Catenin - metabolism Cell Proliferation Cyclin D1 - genetics Cyclin D1 - metabolism Gene Expression Regulation, Bacterial HT29 Cells Humans Lactobacilli Lactobacillus - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Notch RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Signal Transduction Transcription Factor HES-1 - genetics Transcription Factor HES-1 - metabolism Wnt Signaling Pathway Wnt/β-catenin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Probiotics could be considered as attractive candidates for preventing tumor growth through maintaining homeostasis. The aim of this study was to evaluate the inhibitory effect of a cocktail of five Lactobacillus species on human colorectal carcinoma cell line HT-29. The anti-proliferative and apoptotic effects of Lactobacilli cocktail were evaluated using MTT and flow cytometry tests, respectively. Quantitative real-time polymerase chain reaction (qPCR) was used to analyze the expression of several genes in the Notch (notch, hes1, msi1, and numb) and Wnt/β-catenin (CTNNB1 and CCND1) pathways, following the treatment of HT-29 cells with Lactobacilli cocktail. The treatment by Lactobacilli cocktail induced a significant anti-proliferative effect and late stage apoptosis among the cancer cells (p
ISSN:	0882-4010 1096-1208
DOI:	10.1016/j.micpath.2019.103829