Effect of sperm selection method by cumulus oophorus complexes and conventional sperm preparation method on sperm quality and DNA fragmentation for assisted reproduction techonology

To compare the effectiveness of sperm selection using cumulus oophorus complexes (COCs) and conventional sperm preparation methods on sperm quality and DNA fragmentation Normal semen samples under the World Health Organization (WHO)’s 2010 eligibility criteria were collected and processed using conventional sperm preparation methods. The prepared sperm were divided into two groups. Spermatozoa in the study group were selected based on their ability to penetrate a layer of COCs. In the control group, spermatozoa were kept in culture medium under similar conditions. The selected-sperm were evaluated based on sperm quality and DNA fragmentation. Thirty normal semen samples were recruited. Spermatozoa that were able to passthrough the COCs had significantly higher sperm motility parameters than the control group (curvilinear velocity [VCL; 143.5 vs 122.2; P