Human Endometrial Transcriptome and Progesterone Receptor Cistrome Reveal Important Pathways and Epithelial Regulators
Abstract Context Poor uterine receptivity is one major factor leading to pregnancy loss and infertility. Understanding the molecular events governing successful implantation is hence critical in combating infertility. Objective To define Progesterone Receptor (PGR)-regulated molecular mechanisms and...
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Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2020-04, Vol.105 (4), p.e1419-e1439, Article 117 |
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Sprache: | eng |
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Zusammenfassung: | Abstract
Context
Poor uterine receptivity is one major factor leading to pregnancy loss and infertility. Understanding the molecular events governing successful implantation is hence critical in combating infertility.
Objective
To define Progesterone Receptor (PGR)-regulated molecular mechanisms and epithelial roles in receptivity.
Design
RNA-sequencing and PGR-ChIP-seq were conducted in parallel to identify PGR-regulated pathways during the Window of implantation (WOI) in endometrium of fertile women.
Setting
Endometrial biopsies from the proliferative and mid-secretory phases were analyzed.
Patients or Other Participants
Participants were fertile, reproductive aged (18–37 years) women with normal cycle length, and without any history of dysmenorrhea, infertility, or irregular cycles. In total, 42 endometrial biopsies obtained from 42 women were analyzed in this study.
Interventions
There were no interventions during this study.
Main Outcome Measures
Here we measured the alterations in gene expression and PGR occupancy in the genome during the WOI, based on the hypothesis that PGR binds uterine chromatin cycle dependently to regulate genes involved in uterine cell differentiation and function.
Results
653 genes were identified with regulated PGR binding and differential expression during the WOI. These were involved in regulating inflammatory response, xenobiotic metabolism, epithelial mesenchymal transition, cell death, interleukin/Signal Transducer And Activator Of Transcription (STAT) signaling, estrogen response, and Mammalian target of rapamycin complex 1 (MTORC1) response. Transcriptome of the epithelium identified 3052 differentially expressed genes, of which 658 were uniquely regulated. Transcription factors Interferon Regulatory Factor 8 (IRF8) and Myocyte Enhancer Factor 2C (MEF2C) were found to be regulated in the epithelium during the WOI at the protein level, suggesting potentially important functions that are previously unrecognized.
Conclusion
PGR binds the genomic regions of genes regulating critical processes in uterine receptivity and function. |
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ISSN: | 0021-972X 1945-7197 1945-7197 |
DOI: | 10.1210/clinem/dgz117 |