Characteristics of song, brain-anatomy and blood androgen levels in spontaneously singing female canaries

Females of many northern temperate songbird species sing sporadically. However, detailed descriptions of female song are rare. Here we report a detailed analysis of song in a small number of spontaneously-singing female domesticated canaries (Serinus canaria) under non-breeding, laboratory conditions in a large population of domesticated birds. In-depth analysis showed that these females sang rarely, and the spontaneous songs varied between and within birds over time. Furthermore, spontaneous female songs were distinct from songs of testosterone-induced singing female canaries and from songs of male canaries in both temporal and spectral features. Singing females had significantly elevated plasma androgen levels and a larger size of the major song controlling brain nuclei HVC (used as a proper name) and the robust nucleus of the arcopallium (RA) than non-singing females housed under similar conditions. The sporadically observed production of song and accompanying differences in brain anatomy in female canaries may thus depend on minute intraspecific differences in androgen levels. •Long-term monitoring revealed spontaneous song in female canaries.•Approximately 5% of female canaries sang spontaneously.•Large between and within bird variation in female canary songs.•Singing females had significantly elevated plasma androgen levels.•Spontaneous female songs were distinct from songs of breeding males and testosterone-implanted females.