Long-read nanopore sequencing-based draft genome of a carbapenem-resistant Pseudomonas aeruginosa isolate

•Genome sequencing of carbapenem-resistant P. aeruginosa using MiSeq short-read and Oxford Nanopore long-read sequencing.•The resulting assembly is highly contiguous, containing a total of 6624003bp with three contigs.•Annotation identified 6389 protein-coding genes, of which 6241 (97.7%) were annotated in at least one protein database. Pseudomonas aeruginosa is a common Gram-negative bacterium causing various serious infections, such as lower respiratory tract infection and urinary tract infection in catheterised patients. Here we report the draft genome sequence of a carbapenem-resistant P. aeruginosa (CRPA) isolate. The genome of the CRPA isolate was sequenced using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly was highly contiguous, containing a total of 6624003bp with a GC content of 66.21%. Annotation identified 6389 protein-coding genes. Mutations in the oprD and mexR genes conferred resistance to carbapenems in the CRPA isolate. The draft genome sequence of this CRPA isolate could provide a solid basis for further research on the resistance mechanisms and the development of drug therapy for drug resistance genes.