Chemical constituents and synergistic anti-gout studies on Eurycoma longifolia and potential mechanisms evaluation based on systemic analysis approach

[Display omitted] •E. longifolia could significantly inhibit the joint swelling in rat.•The active ingredients against gout in E. longifolia were predicted.•The potential mechanisms were studied by systematic analysis.•Five compounds performed better than others, which worked holistically. 37 compounds mainly including triterpenoids with the quassinoid skeleton and β-carboline alkaloids have been isolated from the roots of Eurycoma longifolia Jack (EL), which has been used as traditional medicine for a long history. It has been demonstrated that the total extracts from EL could significantly inhibit the joint swelling in MSU-induced acute gout arthritis rat model at middle and high doses (P