Changes in neural network connectivity in mice brain following exposures to palatable food

•Chocolate cues facilitated satiated chocolate-experienced mice to seek for chocolate.•LFP oscillations in the LHa and NAc were sensitive to chocolate cues.•Altered coherence activity among regions were correlated with hedonic hunger.•Interrelations among the OB, NAc, LHa and HP were crucial for emotional drive to eat. Previously, satiated animals or human subjects can still be motivated to eat by palatable food-associated cues. However, neural circuitries of hedonic hunger have not been well investigated. This study identified neural network connectivities between major brain areas in response to chocolate-associated cues following repeated exposures to chocolate. Adult male Swiss albino ICR mice were anesthetized and implanted with intracranial electrodes in the lateral hypothalamus (LHa), nucleus accumbens (NAc), olfactory bulb (OB) and hippocampus (HP) for local field potential (LFP) recording. LFP oscillations were recorded before and after repeated exposures to chocolate for chocolate experienced group whereas control group was not exposed to chocolate. On testing days, satiated animals were individually put into a place preference-like apparatus with two opposite chambers of chocolate and normal chow scent cues, separately. The results showed that chocolate experienced group significantly increased time spent in chocolate chamber whereas control group did not. One-way ANOVA revealed significant influence of chocolate sessions on LFP spectral powers of multiple frequencies in the LHa (delta, low gamma and high gamma) and NAc (high gamma). Moreover, coherence function analyses also highlighted significant increases in LHa-NAc and LHa-OB, and decrease in LHa-HP coherent activities in response to olfactory cues of chocolate. This study demonstrated modifications of neural network connectivity and associative learning following multiple exposures to palatable food. These findings might explain why energy homeostatic hunger is overridden by hedonic hunger.