Sapientia aquatica gen. nov., sp. nov., isolated from a crater lake

A new aerobic betaproteobacterium, strain SA-152 , was isolated from the water of a crater lake. 16S rRNA gene sequence analysis revealed that strain SA-152 belonged to the family (order ) and was phylogenetically related to S20-91 with 97.09 % and to ULPAs1 with 96.00 % 16S rRNA gene pairwise sequence similarity. Cells of strain SA-152 were rod-shaped, non-motile, oxidase-negative and catalase-positive. Its fatty acid profile was dominated by two fatty acids, C 7 and C , the major respiratory quinones were Q-8 and Q-7, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain SA-152 was 48.3 mol%. The new bacterium can be distinguished from closely related genera , , and based on its non-motile and oxidase-negative cells. On the basis of the phenotypic, chemotaxonomic and genomic data, strain SA-152 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is SA-152 (=DSM 29805 =NCAIM B.02613 ).