Development of an Inexpensive and Rapid Operation Device for High-Throughput Real-Time Quantitative PCR-Based CYP2D6 CNV Genotyping

The CYP2D6 gene is the most well characterized gene involved in drug metabolism and is known to have both gene duplication and deletion variants. We report an optimized method for the determination of copy number variation (CNV) in the CYP2D6 gene by a novel purification process for a real-time quan...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biological & pharmaceutical bulletin 2019/10/01, Vol.42(10), pp.1761-1765
Hauptverfasser:	Imai, Miho, Kisoi, Madoka, Moritsugu, Miwako, Murata, Shigenori, Ichikawa, Atsushi, Kinoshita, Kenji
Format:	Artikel
Sprache:	eng
Schlagworte:	Copy number copy number variation (CNV) CYP2D6 CYP2D6 protein Cytochrome P-450 CYP2D6 - genetics Cytochrome P450 DNA Copy Number Variations Drug metabolism Epidemiology Female Gene deletion Gene duplication Genotype Genotyping High-Throughput Screening Assays - instrumentation High-Throughput Screening Assays - methods Humans pharmacogenomics (PGx) Purification Real-Time Polymerase Chain Reaction - instrumentation Real-Time Polymerase Chain Reaction - methods real-time quantitative PCR saliva Saliva - chemistry water-soluble paper
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The CYP2D6 gene is the most well characterized gene involved in drug metabolism and is known to have both gene duplication and deletion variants. We report an optimized method for the determination of copy number variation (CNV) in the CYP2D6 gene by a novel purification process for a real-time quantitative PCR. This high-throughput low-cost method accurately determines CNV in the CYP2D6 gene enabling reliable estimates of disease prediction in large epidemiological samples.
ISSN:	0918-6158 1347-5215
DOI:	10.1248/bpb.b19-00120