The overexpression of rice ACYL‐CoA‐BINDING PROTEIN2 increases grain size and bran oil content in transgenic rice

Summary As Oryza sativa (rice) seeds represent food for over three billion people worldwide, the identification of genes that enhance grain size and composition is much desired. Past reports have indicated that Arabidopsis thaliana acyl‐CoA‐binding proteins (ACBPs) are important in seed development but did not affect seed size. Herein, rice OsACBP2 was demonstrated not only to play a role in seed development and germination, but also to influence grain size. OsACBP2 mRNA accumulated in embryos and endosperm of germinating seeds in qRT‐PCR analysis, while β‐glucuronidase (GUS) assays on OsACBP2pro::GUS rice transformants showed GUS expression in embryos, as well as the scutellum and aleurone layer of germinating seeds. Deletion analysis of the OsACBP2 5’‐flanking region revealed five copies of the seed cis‐element, Skn‐I‐like motif (−1486/−1482, −956/−952, −939/−935, −826/−822, and −766/−762), and the removal of any adversely affected expression in seeds, thereby providing a molecular basis for OsACBP2 expression in seeds. When OsACBP2 function was investigated using osacbp2 mutants and transgenic rice overexpressing OsACBP2 (OsACBP2‐OE), osacbp2 was retarded in germination, while OsACBP2‐OEs performed better than the wild‐type and vector‐transformed controls, in germination, seedling growth, grain size and grain weight. Transmission electron microscopy of OsACBP2‐OE mature seeds revealed an accumulation of oil bodies in the scutellum cells, while confocal laser scanning microscopy indicated oil accumulation in OsACBP2‐OE aleurone tissues. Correspondingly, OsACBP2‐OE seeds showed gain in triacylglycerols and long‐chain fatty acids over the vector‐transformed control. As dietary rice bran contains beneficial bioactive components, OsACBP2 appears to be a promising candidate for enriching seed nutritional value. Significance Statement OsACBP2‐OE transgenic rice performed better than the wild‐type and vector‐transformed control, in seedling growth, grain size and weight and transmission electron microscopy detected the accumulation of oil bodies in the OsACBP2‐OE scutellum cells, while lipid profiling of mature OsACBP2‐OE seeds revealed gains in triacylglycerols and long‐chain fatty acids over the vector‐transformed control. As dietary rice bran contains beneficial bioactive components, OsACBP2 appears to be a promising candidate for enriching seed nutritional value.