A sensitive quantification approach for detection of HETE-CP adduct after benzyl chloroformate derivatization using ultra-high-pressure liquid chromatography tandem mass spectrometry

Sulfur mustard (HD) reacts with human serum albumin (HSA) at Cys 34 and produces a long-term biomarker of HD exposure. Here, we present a novel, sensitive, and convenient method for quantification of HD exposure by detection of HD-HSA adducts using pronase digestion, benzyl chloroformate (Cbz-Cl) de...

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Veröffentlicht in:	Analytical and bioanalytical chemistry 2019-06, Vol.411 (15), p.3405-3415
Hauptverfasser:	Chen, Bo, Yu, Hui-Lan, Liu, Shi-Lei, Liu, Chang-Cai, Liang, Long-Hui, Li, Xin-Hai, Li, Xiao-Sen, Wu, Ji-Na, Yang, Yang
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetone Adducts Alkylation Analytical Chemistry Biochemistry Biomarkers Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Chloroformate Chromatography derivatization detection limit dipeptides enzymatic hydrolysis Exposure Food Science High performance liquid chromatography Human serum albumin Laboratory Medicine Liquid chromatography Mass spectrometry Mass spectroscopy Monitoring/Environmental Analysis Mustard gas Pressure Pronase Purification Research Paper retrospective studies Scientific imaging serine proteinases Serum albumin solid phase extraction Solid phases Spectroscopy standard deviation Sulfides Sulfur tandem mass spectrometry
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