MicroRNA signature of human blood mononuclear cells

MicroRNA signature of human blood mononuclear cells

MicroRNAs (miRNAs) regulate a wide range of cellular processes and functions. Blood mononuclear cells (BMNCs) participate in the immune response, inflammatory reaction and angiogenesis. In 2010, a total of 157 miRNAs were quantified by RT-qPCR and a miRNA signature was determined for human periphera...

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Veröffentlicht in:Molecular and cellular biochemistry 2019-12, Vol.462 (1-2), p.167-172
Hauptverfasser: Zhang, Qiuwang, Cannavicci, Anthony, Dai, Si-Cheng, Wang, Chenxi, Kutryk, Michael J. B.
Format: Artikel
Sprache:eng
Schlagworte:
Analysis
Arrays
Biochemistry
Biomedical and Life Sciences
Blood
Cardiology
Centrifugation
Gene Expression Profiling
Gene Expression Regulation
Gene sequencing
Humans
Immune response
Leukocytes, Mononuclear - metabolism
Life Sciences
Medical Biochemistry
MicroRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
Oncology
Ribonucleic acid
RNA
RNA sequencing
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Zusammenfassung:MicroRNAs (miRNAs) regulate a wide range of cellular processes and functions. Blood mononuclear cells (BMNCs) participate in the immune response, inflammatory reaction and angiogenesis. In 2010, a total of 157 miRNAs were quantified by RT-qPCR and a miRNA signature was determined for human peripheral BMNCs. With the advent of technologies such as RNA sequencing, many new miRNAs have been identified. This study was designed to provide an up-to-date miRNA signature for human BMNCs. Peripheral BMNCs were isolated by Ficoll density gradient centrifugation. Using the qPCR array assay, we identified 108 highly expressed miRNAs (Ct value
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-019-03619-3