Identification of bifucosylated glycoforms using low‐energy CID spectra

Identification of bifucosylated glycoforms using low‐energy CID spectra

We have used tandem mass spectrometry (MS/MS)‐based analysis of glycopeptides in order to identify the composition and structure of rare glycoforms. The results illustrate utility of low‐energy MS/MS for structure identification. We have shown the presence of bifucosylated and trifucosylated glycofo...

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Veröffentlicht in:Journal of mass spectrometry. 2019-10, Vol.54 (10), p.817-822
Hauptverfasser: Ács, András, Turiák, Lilla, Révész, Ágnes, Vékey, Károly, Drahos, László
Format: Artikel
Sprache:eng
Schlagworte:
Antennae
Antibiotics
Chromatography, High Pressure Liquid
Fucose - chemistry
fucosylation
glycopeptide
Glycopeptides
Glycopeptides - analysis
Glycopeptides - chemistry
Glycoproteins
Glycosylation
Identification
low‐energy CID
Mass spectrometry
Mass spectroscopy
Molecular Conformation
Orosomucoid - chemistry
Tandem Mass Spectrometry
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Zusammenfassung:We have used tandem mass spectrometry (MS/MS)‐based analysis of glycopeptides in order to identify the composition and structure of rare glycoforms. The results illustrate utility of low‐energy MS/MS for structure identification. We have shown the presence of bifucosylated and trifucosylated glycoforms in human α‐1‐acid glycoprotein (AGP), a major plasma glycoprotein. Fucosylation in the case of AGP always occurs on the antennae; core fucosylation was not observed.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.4432