Lactobacillus huachuanensis sp. nov., isolated from Chinese traditional pickle

A Gram-stain-positive bacterial strain, 395-6.2 , was isolated from traditional pickle in Heilongjiang Province, PR China. The bacterium was characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, DNA-DNA hybridisation ( DDH) and an analysis of phenotypic features. Analysis of the 16S rRNA gene sequence showed that strain 395-6.2 was phylogenetically related to , , , , , , , , , , , , , and . Strain 395-6.2 exhibited 95.7-99.4 % 16S rRNA gene sequence similarities, 85.0-94.0 % gene sequence similarities, 94.2-98.0 % gene sequence similarities to type strains of phylogenetically related species. ANI and DDH values between strain 395-6.2 and type strains of phylogenetically related species were 77.9-87.1 % and 22.5-33.5 %, respectively. Based upon the data obtained in the present study, a novel species, sp. nov., is proposed and the type strain is 395-6.2 (=CCM 8927 =NCIMB 15188 =LMG 31179 ).