Ruegeria lutea sp. nov., isolated from marine sediment, Masan Bay, South Korea

A Gram-stain-negative, non-motile, mesophilic, short rod-shaped, aerobic bacterium designated as 318-1 was isolated from a marine sediment collected from Masan Bay, South Korea. Strain 318-1 grew optimally at pH 6-7, at 30 °C and in the presence of 2-3 % (w/v) NaCl, tolerant of up to 8 % (w/v) NaCl, and accumulated poly- -hydroxybutyrate (PHB). A comparative analysis of 16S rRNA gene sequences revealed that strain 318-1 formed a distinct phyletic lineage in the genus (family , class ) and showed high sequence similarity to DSM 27839 (96.5 %) and DSM 28453 (96.3 %). Comparing the genome sequence of 318-1 with those of the type strains of seven species of the genus and two species of the genus the values obtained were below the thresholds with analysis of average nucleotide identities (ANI, 71.6-76.8 %) and DNA-DNA hybridisation, Genome-to-Genome Distance Calculator (GGDC, 18.5-20.6 %). The DNA G C content was 65.75 mol%. Chemotaxonomic data [predominant quinone ubiquinone Q10; polar lipid profile consisting of major compounds phosphatidylcholine (PC), phosphatidylglycerol (PG), an unidentified aminolipid and an unidentified lipid; major fatty acids summed feature 8 (C ω and/or C ω )] supported the affiliation of strain 318-1 to the genus . Genomic, chemotaxonomic, and phenotypic differentiation of strain 318-1 from the members of the genus support it as a novel species. On the basis of the results in this study, a novel species, sp. nov., is proposed. The type strain is 318-1 (=JCM 30927 =KEMB 7306-525 =KCTC 72105 ).