Effect of salinity fluctuation on the transcriptome of the Japanese mantis shrimp Oratosquilla oratoria

Salinity fluctuation may detrimentally affect the composition and biological processes of crustaceans. As a euryhaline crustacean, Oratosquilla oratoria can survive at salinities ranging from 20 psu to 40 psu. Therefore, we designed five salinity gradients (20, 25, 30, 35, and 40 psu) and 66.39 Gb clean transcriptome data were obtained after O. oratorias were exposed to each gradient for 24 h. All clean data were spliced into 50,482 unigenes and 17,035 unigenes were annotated in at least one database. Compared with 30 psu, 1010, 851, 1733 and 2188 differentially expressed genes were obtained at 20, 25, 35 and 40 psu, respectively. Results also showed that the osmoregulation of O. oratoria is primarily regulated by lipid and amino acid metabolism, amongst others. No significant up-regulated pathways were enriched at 25 psu and 35 psu, although more significant down-regulated pathways were obtained at 35 psu. Therefore, we assumed that the optimum survival salinity of O. oratoria may range from 25 psu to 35 psu. However, 35 psu may be more suitable for O. oratoria. In addition, 55 unigenes that encode putative inorganic ion exchanges were identified. This study aims to provide fundamental information for understanding the osmoregulation mechanisms of crustaceans.