A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values t...

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Veröffentlicht in:Journal of microbiological methods 2019-10, Vol.165, p.105703-105703, Article 105703
Hauptverfasser: Macori, Guerrino, McCarthy, Siobhán C., Burgess, Catherine M., Fanning, Séamus, Duffy, Geraldine
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Sprache:eng
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Escherichia coli O157
Escherichia coli O26
Quantitative real-time PCR
Sheep
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container_title Journal of microbiological methods
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McCarthy, Siobhán C.
Burgess, Catherine M.
Fanning, Séamus
Duffy, Geraldine
description A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.
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subjects Escherichia coli O157
Escherichia coli O26
Quantitative real-time PCR
Sheep
title A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs
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