A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs

A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values t...

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Veröffentlicht in:	Journal of microbiological methods 2019-10, Vol.165, p.105703-105703, Article 105703
Hauptverfasser:	Macori, Guerrino, McCarthy, Siobhán C., Burgess, Catherine M., Fanning, Séamus, Duffy, Geraldine
Format:	Artikel
Sprache:	eng
Schlagworte:	Escherichia coli O157 Escherichia coli O26 Quantitative real-time PCR Sheep
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Beschreibung
Zusammenfassung:	A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2019.105703