pH-triggered delivery of magnetic nanoparticles depends on tumor volume

Nowadays there is growing recognition of the fact that biological systems have a greater impact on nanoparticle target delivery in tumors than nanoparticle design. Here we investigate the targeted delivery of Fe3O4 magnetic nanoparticles conjugated with pH-low-insertion peptide (MNP-pHLIP) on orthotopically induced MDA-MB-231 human breast carcinoma xenografts of varying volumes as a model of cancer progression. Using in vivo magnetic resonance imaging and subsequent determination of iron content in tumor samples by inductively coupled plasma atomic emission spectroscopy we found that MNP-pHLIP accumulation depends on tumor volume. Transmission electron microscopy, histological analysis and immunohistochemical staining of tumor samples suggest that blood vessel distribution is the key factor in determining the success of the accumulation of nanoparticles in tumors. Iron oxide magnetic nanoparticles conjugated with pH-low-insertion peptide accumulated in orthotopically induced MDA-MB-231 human breast carcinoma xenografts with volume varying from 0.08 to 0.40 cm3 predominantly in dense blood vessel supply regions. [Display omitted]