Discovery of potent p38α MAPK inhibitors through a funnel like workflow combining in silico screening and in vitro validation

This work describes the rational discovery of novel chemotypes of p38α MAPK inhibitors using a funnel approach consisting of several computer-aided drug discovery methods and biological experiments. Among the identified hits, four compounds belonging to different chemical families showed IC50 values lower than 10 μM. In particular, the 1,4-benzodioxane derivative 5 turned out to be a potent and efficient p38α MAPK inhibitor having IC50 = 0.07 μM, and LEexp and LipE values of 0.38 and 4.8, respectively; noteworthy, the compound had also a promising kinase selectivity profile and the capability to suppress p38α MAPK effects in human immune cells. Overall, the collected findings highlight that the applied strategy has been successful in generating chemical novelty in the inhibitor kinase field, providing suitable chemical candidates for further inhibitor optimization. [Display omitted] •Drugs active against p38α MAPK have not yet been in the market.•A funnel-like strategy aided the discovery of novel chemotypes of p38α MAPK inhibitors.•Compound 5 had IC50 = 0.07 μM and suppressed p38α MAPK effects in human immune cells.