Chryseobacterium mulctrae sp. nov., isolated from raw cow's milk

A Gram-stain-negative bacterial strain, designated CA10 , was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysi...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2019-11, Vol.69 (11), p.3478-3484
Hauptverfasser: Yoon, Sung-Hee, Lee, Ju-Eun, Han, Rae-Hee, Kwon, Mirae, Kim, Geun-Bae
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Sprache:eng
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Zusammenfassung:A Gram-stain-negative bacterial strain, designated CA10 , was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain CA10 belonged to the genus , family , and was most closely related to ATCC 27950 (98.75 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values of strain CA10 were 94.4 and 56.9 %, respectively, relative to DSM 16779 , being lower than the cut-off values of 95-96 and 70 %, respectively. The predominant respiratory quinone was menaquinone-6; major polar lipid, phosphatidylethanolamine; major fatty acids, iso-C , summed feature 9 (iso-C ω9 and/or C 10-methyl), summed feature 3 (iso-C 2-OH and/or C ω7 ) and iso-C 3-OH. The results of physiological, chemotaxonomic and biochemical analyses suggested that strain CA10 is a novel species of genus , for which the name sp. nov. is proposed. The type strain is CA10 (=KACC 21234 =JCM 33443 ).
ISSN:1466-5026
1466-5034
DOI:10.1099/ijsem.0.003647