Chryseobacterium mulctrae sp. nov., isolated from raw cow's milk
A Gram-stain-negative bacterial strain, designated CA10 , was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysi...
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Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2019-11, Vol.69 (11), p.3478-3484 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A Gram-stain-negative bacterial strain, designated CA10
, was isolated from bovine raw milk sampled in Anseong, Republic of Korea. Cells were yellow-pigmented, aerobic, non-motile bacilli and grew optimally at 30 °C and pH 7.0 on tryptic soy agar without supplementation of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain CA10
belonged to the genus
, family
, and was most closely related to
ATCC 27950
(98.75 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values of strain CA10
were 94.4 and 56.9 %, respectively, relative to
DSM 16779
, being lower than the cut-off values of 95-96 and 70 %, respectively. The predominant respiratory quinone was menaquinone-6; major polar lipid, phosphatidylethanolamine; major fatty acids, iso-C
, summed feature 9 (iso-C
ω9
and/or C
10-methyl), summed feature 3 (iso-C
2-OH and/or C
ω7
) and iso-C
3-OH. The results of physiological, chemotaxonomic and biochemical analyses suggested that strain CA10
is a novel species of genus
, for which the name
sp. nov. is proposed. The type strain is CA10
(=KACC 21234
=JCM 33443
). |
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ISSN: | 1466-5026 1466-5034 |
DOI: | 10.1099/ijsem.0.003647 |