Individual differences in behavior and heart rate variability across the preweaning period in the domestic horse in response to an ecologically relevant stressor

The study of individual differences in behavior and physiology has attracted considerable interest among behavioral biologists. Important questions include how early in life such differences emerge and to what extent they remain stable across development. Due to the demanding nature of longitudinal studies, there is still a lack of information on this in mammals, especially in large, long-lived species. Our aim in this study was to look for stable individual differences in behavior and physiology during early development in the domestic horse and for correlations between the two parameters. We tested 30 Azteca-breed foals kept under standard conditions by briefly separating them from their mother in four repeated tests beginning at the first postnatal week until the foals were six months old, before they were weaned. Individual differences in behavior and heart rate variability of foals in response to brief maternal separations were consistent and were correlated from a very early age. These findings contribute to an understanding of the expression of individual differences in physiology and behavior from a developmental perspective and may help in the future selection of horses for functional contexts that require different levels of reactivity, thereby contributing to horse welfare and to human safety and economy. •Horses show stable individual differences in stress responses from birth to weaning.•Individual differences in behavior and heart rate under stress are correlated.•Repeated separation of foals from mothers is a suitable method to demonstrate this.•Vocalization is a useful indicator of individual differences in response to stress.•These differences early in development might predict differences in later life.