Evaluating the genotoxic damage in bovine whole blood cells in vitro after exposure to thiacloprid

Evaluating the genotoxic damage in bovine whole blood cells in vitro after exposure to thiacloprid

Possible genotoxic effect of thiacloprid on bovine cultures of whole blood was investigated using chromosomal aberrations (CAs), micronuclei (MN), sister chromatid exchanges (SCEs), DNA damage and apoptotic DNA fragmentation assays. The cells of whole blood were exposed to thiacloprid (30, 60, 120,...

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Veröffentlicht in:Toxicology in vitro 2019-12, Vol.61, p.104616-104616, Article 104616
Hauptverfasser: Galdíková, Martina, Holečková, Beáta, Šiviková, Katarína, Schwarzbacherová, Viera, Koleničová, Simona
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Apoptosis
Bioassays
Blood Cells
Cattle
Cell cycle
Cell proliferation
Cell Proliferation - drug effects
Chromosome Aberrations
Comet assay
Cultivation
Cytotoxicity
Damage detection
Deoxyribonucleic acid
DNA
DNA Damage
DNA fragmentation
Exposure
Genotoxicity
Insecticides - toxicity
Micronuclei
Micronuclei, Chromosome-Defective
Mutagenicity Tests
Mutagens - toxicity
Neonicotinoids - toxicity
Sister Chromatid Exchange
Statistical analysis
Thiacloprid
Thiazines - toxicity
Toxicity
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Zusammenfassung:Possible genotoxic effect of thiacloprid on bovine cultures of whole blood was investigated using chromosomal aberrations (CAs), micronuclei (MN), sister chromatid exchanges (SCEs), DNA damage and apoptotic DNA fragmentation assays. The cells of whole blood were exposed to thiacloprid (30, 60, 120, 240 and 480 μg mL−1) for the last 24 and 48 h of cultivation. Thiacloprid did not induce significant increase in CAs after 24 and 48 h; only the concentration of 120 μg mL−1 caused elevation of CAs (p 
ISSN:0887-2333
1879-3177
DOI:10.1016/j.tiv.2019.104616