Evaluation of mitogenome sequence concordance, heteroplasmy detection, and haplogrouping in a worldwide lineage study using the Precision ID mtDNA Whole Genome Panel

Highlights•The Precision ID mtDNA Whole Genome Panel was evaluated in a large lineage study using different tissues. •Samples included high quality reference samples and also challenging forensic samples. •The vast majority of samples yielded full mitogenomes from diverse phylogenetic backgrounds. •We observed very high concordance to previous Sanger and other MPS-based analyses. •We observed differences in the display of heteroplasmy that deserve further research.