Mucilaginibacter terrigena sp. nov. sp., A Novel Member of the Family Sphingobacteriaceae

A bacterial strain, 17JY9-4 T , was isolated from a soil sample collected on Jeju Island, South Korea. Colonies grown on R2A agar are pale pink in color, and cells are Gram-stain negative, short, and rod-shaped. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Mucilaginibacter in the family Sphingobacteriaceae , with high levels of 16S rRNA sequence similarity shared with Mucilaginibacter lutimaris BR-3 T (98.0%), Mucilaginibacter rigui WPCB133 T (98.0%), Mucilaginibacter phyllosphaerae PP-F2F-G21 T (97.0%), Mucilaginibacter amnicola TAPP7 T (96.8%), and Mucilaginibacter soli R9-65 T (96.7%). Growth of strain 17JY9-4 T occurs at 10–30 °C, pH 6–8, and in the presence of 0–1.0% NaCl. The genomic G+C content is 44.38 mol%. The predominant respiratory quinone of the isolate is MK-7; the major fatty acids are summed feature 3 (C 16:1 ω 7 c /C 16:1 ω 6 c ) (39.7%), iso-C 15:0 (22.8%), iso-C 17:0 3-OH (7.8%), and C 16:0 (7.7%); and the major polar lipid is phosphatidylethanolamine. The phenotypic and chemotaxonomic data support the placement of strain 17JY9-4 T within the genus Mucilaginibacter . However, the DNA–DNA relatedness between the isolate and M. rigui , M. lutimaris , M. phyllosphaerae , M. amnicola , and M. soli were 44.3 ± 3.0%, 38.6 ± 3.7%, 23.2 ± 2.9%, 21.9 ± 3.1%, and 18.6 ± 3.7%, respectively. The results of 16S rRNA gene sequence similarity analysis, DNA–DNA hybridization analysis, and the observed differentiating phenotypic properties from other closely related taxa clearly indicate that strain 17JY9-4 T represents a novel species in the genus Mucilaginibacter , for which the name Mucilaginibacter terrigena sp. nov. is proposed. The type strain is 17JY9-4 T (= KCTC 62294 T = JCM 33049 T ).