Total Amino Acids by UHPLC–UV in Infant Formulas and Adult Nutritionals, First Action 2018.06
Background: An acid hydrolysis ultrahigh-performance LC--UV method was evaluated for the determination of total amino acids in infant formula and adult/pediatric nutritional formula. Objective: It was assessed for compliance against AOAC INTERNATIONAL Standard Method Performance Requirements (SMPR[R...
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Veröffentlicht in: | Journal of AOAC International 2019-09, Vol.102 (5), p.1574-1588 |
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Sprache: | eng |
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Zusammenfassung: | Background: An acid hydrolysis ultrahigh-performance LC--UV method was evaluated for the determination of total amino acids in infant formula and adult/pediatric nutritional formula. Objective: It was assessed for compliance against AOAC INTERNATIONAL Standard Method Performance Requirements (SMPR[R]) established by the stakeholder Panel for Infant Formula and Adult Nutritionals (SPIFAN). Methods: A single-laboratory validation (SLV) study was conducted as a first step in the process to validate the method. In this SLV, 17 SPIFAN matrices representing a range of infant formula and adult nutritional products were evaluated for their amino acid content. Results: The analytical range was found to be within the needs for all products; some may require a dilution. Evaluation of trueness performed on standard Reference Material 1849a (Infant/Adult Nutritional Formula) showed all compounds met the SMPR theoretical value, with exceptions for threonine and tyrosine. These may have a bias for the National Institute of standards and Technology (NIST) data, depending on hydrolysis used in the determination of the NIST certificate of analysis. Conclusions: Based on the results of this SLV, this method met the SMPR and was approved as a First Action method by the AOAC Expert Review Panel on Nutrient Methods on August 28, 2018. |
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ISSN: | 1060-3271 1944-7922 |
DOI: | 10.5740/jaoacint.19-0036 |