Lamb wave-based molecular diagnosis using DNA hydrogel formation by rolling circle amplification (RCA) process

Recent developments in microfluidics enable the lab-on-a-chip-based molecular diagnosis. Rapid and accurate diagnosis of infectious diseases is critical for preventing the transmission of the disease. Here, we characterize a Lamb wave-based device using various parameters including the contact angle...

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Veröffentlicht in:Biosensors & bioelectronics 2019-10, Vol.142, p.111496-111496, Article 111496
Hauptverfasser: Nam, Jeonghun, Jang, Woong Sik, Kim, Jisu, Lee, Hyukjin, Lim, Chae Seung
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Sprache:eng
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Zusammenfassung:Recent developments in microfluidics enable the lab-on-a-chip-based molecular diagnosis. Rapid and accurate diagnosis of infectious diseases is critical for preventing the transmission of the disease. Here, we characterize a Lamb wave-based device using various parameters including the contact angle and viscosity of the sample droplet, the applied voltage, and the temperature increase. Additionally, we demonstrate the functionality of the Lamb wave-based device in clinical application. Optimal temperature for rolling circle amplification (RCA) process is 30 °C, and it was achieved by Lamb wave generation at 17 V. Gene amplification due to RCA process could be detected by viscosity increase due to DNA hydrogel formation in a sample droplet, which induced the acoustic streaming velocity of suspended particles to be decreased. In our Lamb wave-based device, isothermal amplification of target nucleic acids could be successfully detected within 30 min using 10 μL of sessile droplet, and was validated by comparing that of commercial real-time fluorescence analysis. Our device enables simple and low-cost molecular diagnosis, which can be applied to resource-limited clinical settings. •During rolling circle amplification (RCA) process, the viscosity increases due to hydrogel formation in a sample droplet.•Lamb wave-induced acoustic streaming velocity in a sample droplet decreases with viscosity increase.•Optimal temperature for RCA process is 30 °C, and it was achieved by Lamb wave generation at 17 V.•In our device, RCA products can be detected by using small volume of samples (10 μL) in short time duration (~ 30 min).•Lamb wave-based detection of DNA amplification was validated by RCA real-time fluorescence analysis using dengue virus.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2019.111496