3D in vivo imaging of the keratin filament network in the mouse stratum granulosum reveals profilaggrin-dependent regulation of keratin bundling

•We visualized keratin filament networks in epidermal stratum granulosum in vivo.•mCherry-keratin 1 labeled keratin filaments without affecting filament network.•mCherry-K1 was expressed in stratum granulosum by in vivo electroporation.•Filaggrin-deficient stratum granulosum exhibited reduced bundle...

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Veröffentlicht in:	Journal of dermatological science 2019-06, Vol.94 (3), p.346-349
Hauptverfasser:	Usui, Keiko, Kadono, Nanako, Furuichi, Yuki, Shiraga, Keiichiro, Saitou, Takashi, Kawasaki, Hiroshi, Toyooka, Kiminori, Tamura, Hiroomi, Kubo, Akiharu, Amagai, Masayuki, Matsui, Takeshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Epidermis - diagnostic imaging Epidermis - ultrastructure Filaggrin Proteins Genes, Reporter - genetics Green Fluorescent Proteins - genetics HeLa Cells Humans Imaging, Three-Dimensional - methods Intermediate Filament Proteins - genetics Intermediate Filament Proteins - metabolism Intermediate Filaments - metabolism Intermediate Filaments - ultrastructure Intravital Microscopy - methods Keratinocytes - metabolism Keratinocytes - ultrastructure Keratins - metabolism Keratins - ultrastructure Luminescent Proteins - genetics Male Mice Mice, Hairless Mice, Knockout Microscopy, Confocal Microscopy, Electron Primary Cell Culture Red Fluorescent Protein S100 Proteins - metabolism
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Zusammenfassung:	•We visualized keratin filament networks in epidermal stratum granulosum in vivo.•mCherry-keratin 1 labeled keratin filaments without affecting filament network.•mCherry-K1 was expressed in stratum granulosum by in vivo electroporation.•Filaggrin-deficient stratum granulosum exhibited reduced bundled keratin filaments.
ISSN:	0923-1811 1873-569X
DOI:	10.1016/j.jdermsci.2019.04.006