Shedding Light into the Subcutis: A Mass Spectrometry Based Immunocapture Assay Enabling Full Characterization of Therapeutic Antibodies after Injection in Vivo

An understanding of what happens to therapeutic antibodies in vivo after subcutaneous injection is of high interest. Therefore, we applied the open flow microperfusion technique to extract interstitial fluid from the subcutaneous tissue. In order to analyze those biological samples, a specific and s...

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Veröffentlicht in:	Analytical chemistry (Washington) 2019-08, Vol.91 (15), p.9490-9499
Hauptverfasser:	Doell, Annika, Schmitz, Oliver J, Hollmann, Markus
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Beads Biological properties Biological samples Chemistry Desalination Immunoglobulins Injection Mass spectrometry Mass spectroscopy Monoclonal antibodies Peptides Quality management Streptavidin Workflow
Online-Zugang:	Volltext
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