Luminescent carbon nanodots based aptasensors for rapid detection of kanamycin residue

Despite the success in long-term storage of food and dietary products using antibiotics as supplements, enormous levels of their residues have remained as a significant health concern, leading to severe toxicity issues on consumption. Herein, we report an ultrasensitive and highly selective aptasensor based on carbon nanoparticles (CNPs) through a fluorescence-based aptamer-linked immunosorbent assay (FALIA) for rapid detection of kanamycin (KAA) residue. The fabricated CNP-aptasensor exhibited superior selectivity with exceptional photoluminescence properties. Under the optimal conditions, the linear equation of standard KAA solution was Y = −0.2279LogX+1.3648 (R = −0.9893) ranged from 10−4 to 10−7 ppb with excellent relative standard deviations (RSD) between 3.12 and 5.59 % (n = 3). Moreover, the limit of detection (LOD) was lower than 5.0 × 10−8 ppb. Together, the excellent recovery and significant efficacy in the rapid detection of antibiotics at a low level in milk indicate that this fabricated CNP-aptasensor has a great potential in the establishment of an efficient antibiotic detector system in food and other nutraceutical industries. [Display omitted] •A simple and sensitivity method for kanamycin residue assay was developed by carbon nanodots aptasensors and fluorescence based aptamer linked immunosorbent assay.•A carbon nanodots based aptasensors have been fabricated and a simple method for kanamycin residue assay was developed.•The proposed has been successfully employed to analyze kanamycin in milk.