Nitrogen removal from wastewater via simultaneous nitrification and denitrification using a biological folded non-aerated filter

[Display omitted] •Removal of nitrogen by biological folded non-aerated filter reactor was studied.•COD/N ratio and HRT have great influence on the performance of the BFNAF reactor.•Several AOB, NOB and DNB were found to dominate the community of the biofilm.•SND could be realized in the BFNAF reactor. A conventional biological filter has been shown to be a viable method for removing nitrogenous compounds from wastewater, but it still has many disadvantages. In this study, a biological folded non-aerated filter (BFNAF) was designed, and its feasibility for nitrogen-loaded wastewater treatment has been confirmed. Effects of the HRT and the COD/N ratio on the performance of BFNAF were investigated. Through response surface method, when the COD/N ratio and the HRT were 5.39 and 10.83 h, removal efficiencies of NH4+, COD and TN reached maximum values of 88.62 ± 0.81%, 76.12 ± 0.57%, and 50.48 ± 1.02%, respectively. In addition, it was found that several denitrifying bacteria, such as Azoarcus, Arcobacter, Flavobacterium, along with many ammonia-oxidizing bacteria and nitrite-oxidizing bacteria, co-existed in the community of the biofilm. All the results showed that the BFNAF could realize the simultaneous nitrification and denitrification (SND) process effectively.