Secretome of Cultured Human Endothelial Cells in Simulated Microgravity
The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many struc...
Gespeichert in:
| Veröffentlicht in: | Bulletin of experimental biology and medicine 2019-05, Vol.167 (1), p.35-38 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 38 |
|---|---|
| container_issue | 1 |
| container_start_page | 35 |
| container_title | Bulletin of experimental biology and medicine |
| container_volume | 167 |
| creator | Kashirina, D. N. Kononikhin, A. S. Larina, I. M. Buravkova, L. B. |
| description | The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many structural elements of microtubules and regulatory proteins interacting with Rho-GTPases. Hence, reorganization of actin cytoskeleton and microtubules induced by microgravity is under complex regulation mediated by Rho proteins. |
| doi_str_mv | 10.1007/s10517-019-04454-8 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2248380897</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A597962497</galeid><sourcerecordid>A597962497</sourcerecordid><originalsourceid>FETCH-LOGICAL-c473t-45106e22449516768ce8fbdc1060420ef9f8932c46a085ceb0e37ae8ecbf56a63</originalsourceid><addsrcrecordid>eNp9kV9rFDEUxYModq1-AR9kQBBfpiaTTJJ5LEtthYoP1eeQzdzspmSSmj9Cv73ZbrVWRPIQcvM7h3vvQeg1wScEY_EhEzwS0WMy9ZixkfXyCVqRUdBeDgN5ila4UT2TUh6hFzlf75-Yk-foiBIiBON8hc6vwCQocYEu2m5dfakJ5u6iLjp0Z2GOZQfead-twfvcudBduaV6XRr02ZkUt0n_cOX2JXpmtc_w6v4-Rt8-nn1dX_SXX84_rU8ve8MELT0bCeYwDIxNI-GCSwPSbmbTqpgNGOxk5UQHw7jGcjSwwUCFBglmY0euOT1G7w--Nyl-r5CLWlw2rTcdINasmrWkEstJNPTtX-h1rCm07hpF-dRWRtgDtdUelAs2lqTN3lSdjpOY-MDuvE7-QbUzw-JMDGBdqz8SvPtDsAPtyy5HX4uLIT8GhwPYdplzAqtuklt0ulUEq33M6hCzajGru5iVbKI396PVzQLzb8mvXBtAD0BuX2EL6WH2_9j-BLJVruo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2236944514</pqid></control><display><type>article</type><title>Secretome of Cultured Human Endothelial Cells in Simulated Microgravity</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Kashirina, D. N. ; Kononikhin, A. S. ; Larina, I. M. ; Buravkova, L. B.</creator><creatorcontrib>Kashirina, D. N. ; Kononikhin, A. S. ; Larina, I. M. ; Buravkova, L. B.</creatorcontrib><description>The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many structural elements of microtubules and regulatory proteins interacting with Rho-GTPases. Hence, reorganization of actin cytoskeleton and microtubules induced by microgravity is under complex regulation mediated by Rho proteins.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-019-04454-8</identifier><identifier>PMID: 31177466</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Actin ; Actin Cytoskeleton - metabolism ; Analysis ; Arrhythmia ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Chromatography ; Cytoskeleton ; Endothelial cells ; Endothelium ; G proteins ; Guanine nucleotide-binding protein ; Histamine ; Human Umbilical Vein Endothelial Cells - metabolism ; Humans ; Internal Medicine ; Laboratory Medicine ; Mass Spectrometry ; Mass spectroscopy ; Microgravity ; Microtubules ; Microtubules - metabolism ; Muscle proteins ; Pathology ; Proteins ; Regulatory proteins ; rho GTP-Binding Proteins - metabolism ; Secretome ; Signal Transduction - physiology ; Spectroscopy ; Umbilical vein ; Weightlessness Simulation</subject><ispartof>Bulletin of experimental biology and medicine, 2019-05, Vol.167 (1), p.35-38</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Bulletin of Experimental Biology and Medicine is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-45106e22449516768ce8fbdc1060420ef9f8932c46a085ceb0e37ae8ecbf56a63</citedby><cites>FETCH-LOGICAL-c473t-45106e22449516768ce8fbdc1060420ef9f8932c46a085ceb0e37ae8ecbf56a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-019-04454-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-019-04454-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31177466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kashirina, D. N.</creatorcontrib><creatorcontrib>Kononikhin, A. S.</creatorcontrib><creatorcontrib>Larina, I. M.</creatorcontrib><creatorcontrib>Buravkova, L. B.</creatorcontrib><title>Secretome of Cultured Human Endothelial Cells in Simulated Microgravity</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many structural elements of microtubules and regulatory proteins interacting with Rho-GTPases. Hence, reorganization of actin cytoskeleton and microtubules induced by microgravity is under complex regulation mediated by Rho proteins.</description><subject>Actin</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Analysis</subject><subject>Arrhythmia</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Chromatography</subject><subject>Cytoskeleton</subject><subject>Endothelial cells</subject><subject>Endothelium</subject><subject>G proteins</subject><subject>Guanine nucleotide-binding protein</subject><subject>Histamine</subject><subject>Human Umbilical Vein Endothelial Cells - metabolism</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Microgravity</subject><subject>Microtubules</subject><subject>Microtubules - metabolism</subject><subject>Muscle proteins</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Regulatory proteins</subject><subject>rho GTP-Binding Proteins - metabolism</subject><subject>Secretome</subject><subject>Signal Transduction - physiology</subject><subject>Spectroscopy</subject><subject>Umbilical vein</subject><subject>Weightlessness Simulation</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kV9rFDEUxYModq1-AR9kQBBfpiaTTJJ5LEtthYoP1eeQzdzspmSSmj9Cv73ZbrVWRPIQcvM7h3vvQeg1wScEY_EhEzwS0WMy9ZixkfXyCVqRUdBeDgN5ila4UT2TUh6hFzlf75-Yk-foiBIiBON8hc6vwCQocYEu2m5dfakJ5u6iLjp0Z2GOZQfead-twfvcudBduaV6XRr02ZkUt0n_cOX2JXpmtc_w6v4-Rt8-nn1dX_SXX84_rU8ve8MELT0bCeYwDIxNI-GCSwPSbmbTqpgNGOxk5UQHw7jGcjSwwUCFBglmY0euOT1G7w--Nyl-r5CLWlw2rTcdINasmrWkEstJNPTtX-h1rCm07hpF-dRWRtgDtdUelAs2lqTN3lSdjpOY-MDuvE7-QbUzw-JMDGBdqz8SvPtDsAPtyy5HX4uLIT8GhwPYdplzAqtuklt0ulUEq33M6hCzajGru5iVbKI396PVzQLzb8mvXBtAD0BuX2EL6WH2_9j-BLJVruo</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Kashirina, D. N.</creator><creator>Kononikhin, A. S.</creator><creator>Larina, I. M.</creator><creator>Buravkova, L. B.</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20190501</creationdate><title>Secretome of Cultured Human Endothelial Cells in Simulated Microgravity</title><author>Kashirina, D. N. ; Kononikhin, A. S. ; Larina, I. M. ; Buravkova, L. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-45106e22449516768ce8fbdc1060420ef9f8932c46a085ceb0e37ae8ecbf56a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Actin</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Analysis</topic><topic>Arrhythmia</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Biology</topic><topic>Chromatography</topic><topic>Cytoskeleton</topic><topic>Endothelial cells</topic><topic>Endothelium</topic><topic>G proteins</topic><topic>Guanine nucleotide-binding protein</topic><topic>Histamine</topic><topic>Human Umbilical Vein Endothelial Cells - metabolism</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Laboratory Medicine</topic><topic>Mass Spectrometry</topic><topic>Mass spectroscopy</topic><topic>Microgravity</topic><topic>Microtubules</topic><topic>Microtubules - metabolism</topic><topic>Muscle proteins</topic><topic>Pathology</topic><topic>Proteins</topic><topic>Regulatory proteins</topic><topic>rho GTP-Binding Proteins - metabolism</topic><topic>Secretome</topic><topic>Signal Transduction - physiology</topic><topic>Spectroscopy</topic><topic>Umbilical vein</topic><topic>Weightlessness Simulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kashirina, D. N.</creatorcontrib><creatorcontrib>Kononikhin, A. S.</creatorcontrib><creatorcontrib>Larina, I. M.</creatorcontrib><creatorcontrib>Buravkova, L. B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Bulletin of experimental biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kashirina, D. N.</au><au>Kononikhin, A. S.</au><au>Larina, I. M.</au><au>Buravkova, L. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Secretome of Cultured Human Endothelial Cells in Simulated Microgravity</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2019-05-01</date><risdate>2019</risdate><volume>167</volume><issue>1</issue><spage>35</spage><epage>38</epage><pages>35-38</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>The secretome of human umbilical vein endothelial cells (HUVEC) cultured under static conditions and in modeled microgravity for 24 h was studied by chromatography-mass spectrometry. In the secretome of cells exposed to microgravity, we identified a group of microtubule proteins including many structural elements of microtubules and regulatory proteins interacting with Rho-GTPases. Hence, reorganization of actin cytoskeleton and microtubules induced by microgravity is under complex regulation mediated by Rho proteins.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>31177466</pmid><doi>10.1007/s10517-019-04454-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-4888 |
| ispartof | Bulletin of experimental biology and medicine, 2019-05, Vol.167 (1), p.35-38 |
| issn | 0007-4888 1573-8221 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2248380897 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Actin Actin Cytoskeleton - metabolism Analysis Arrhythmia Biomedical and Life Sciences Biomedicine Cell Biology Chromatography Cytoskeleton Endothelial cells Endothelium G proteins Guanine nucleotide-binding protein Histamine Human Umbilical Vein Endothelial Cells - metabolism Humans Internal Medicine Laboratory Medicine Mass Spectrometry Mass spectroscopy Microgravity Microtubules Microtubules - metabolism Muscle proteins Pathology Proteins Regulatory proteins rho GTP-Binding Proteins - metabolism Secretome Signal Transduction - physiology Spectroscopy Umbilical vein Weightlessness Simulation |
| title | Secretome of Cultured Human Endothelial Cells in Simulated Microgravity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T18%3A50%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Secretome%20of%20Cultured%20Human%20Endothelial%20Cells%20in%20Simulated%20Microgravity&rft.jtitle=Bulletin%20of%20experimental%20biology%20and%20medicine&rft.au=Kashirina,%20D.%20N.&rft.date=2019-05-01&rft.volume=167&rft.issue=1&rft.spage=35&rft.epage=38&rft.pages=35-38&rft.issn=0007-4888&rft.eissn=1573-8221&rft_id=info:doi/10.1007/s10517-019-04454-8&rft_dat=%3Cgale_proqu%3EA597962497%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2236944514&rft_id=info:pmid/31177466&rft_galeid=A597962497&rfr_iscdi=true |