Molecular properties and structural characterization of an alkaline extractable arabinoxylan from hull-less barley bran

•An alkali-extracted HBAX-25 from hull-less barley bran was characterized in detail.•The molecular and spectral properties of HBAX-25 were revealed fundamentally.•The structural basis of HBAX-25 was established and confirmed as an arabinoxylan. An alkaline extractable arabinoxylan (HBAX-25) was fractionated from crude arabinoxylan (HBAX) obtained optimally in hull-less barley (Hordeum vulgare L. var. nudum Hook. f.) bran. Molecular properties and structural characterization of HBAX-25 were investigated thoroughly based on chemical composition of 8.31% (w/w) moisture and 87.57% (w/w) sugar with specifically few proteins (1.08%, w/w) and high arabinoxylans (82.46%, w/w). Data from monosaccharide composition indicated that HBAX-25 mainly consisted of arabinose (30.13 mol%) and xylose (51.55 mol%) with A/X ratio of 0.58, representative for arabinoxylans, which coincided with FT-IR results and was corroborated by methylation and NMR analyses, i.e., a relatively low-branched arabinoxylan composed of un-substituted (1,4-linked β-D-Xylp, 71.19%), mono-substituted (1,3,4-linked β-D-Xylp, 14.78%) and di-substituted (1,2,3,4-linked β-D-Xylp, 10.76%) xylose units as backbone via β-(1→4) linkages, with six possible branches or individuals included. Hence, a structural basis of HBAX-25 was established, which could have potential in food and other value-added applications capable of interpreting their physicochemical, functional and technological characteristics.