Robust synthesis of C-terminal cysteine-containing peptide acids through a peptide hydrazide-based strategy

A new robust strategy was reported for the epimerization-free synthesis of C-terminal Cys-containing peptide acids through mercaptoethanol-mediated hydrolysis of peptide thioesters prepared in situ from peptide hydrazides. This simple-to-operate and highly efficient method avoids the use of derivatization reagents for resin modification, thus providing a practical avenue for the preparation of C-terminal Cys-containing peptide acids. A simple-to-operate and highly efficient strategy for the epimerization-free synthesis of C-terminal Cys-containing peptide acids, which avoids the use of derivatization reagents for resin modification, is developed.