MicroRNA miR-31 targets SIRT3 to disrupt mitochondrial activity and increase oxidative stress in oral carcinoma

MicroRNA miR-31 is implicated in the neoplastic process of various malignancies including oral squamous cell carcinoma (OSCC). Silent information regulator 3 (Sirtuin3 or SIRT3) is a NAD-dependent deacetylase that regulates metabolic process. Suppressor role of SIRT3 has been found in neoplasms. Thi...

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Veröffentlicht in:Cancer letters 2019-08, Vol.456, p.40-48
Hauptverfasser: Kao, Yu-Yu, Chou, Chung-Hsien, Yeh, Li-Yin, Chen, Yi-Fen, Chang, Kuo-Wei, Liu, Chung-Ji, Fan Chiang, Chun-Yu, Lin, Shu-Chun
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Sprache:eng
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Zusammenfassung:MicroRNA miR-31 is implicated in the neoplastic process of various malignancies including oral squamous cell carcinoma (OSCC). Silent information regulator 3 (Sirtuin3 or SIRT3) is a NAD-dependent deacetylase that regulates metabolic process. Suppressor role of SIRT3 has been found in neoplasms. This study investigates the disruptions of miR-31-SIRT3 cascade to explore their potential association with metabolic change in OSCC. We identified that miR-31 directly targeted SIRT3 in OSCC cells, and a reverse correlation between miR-31 expression and SIRT3 expression was noted in OSCC tumors. SIRT3 expression attenuated the miR-31 enhanced tumor cell migration and invasion. It also reduced the tumorigenic potential of FaDu cell line. miR-31-SIRT3 impaired the mitochondrial membrane potential and structural integrity. The dis-regulation of this axis also contributed to the genesis of oxidative stress. In addition, miR-31 switched tumor cells from aerobic metabolism to glycolytic metabolism. This study provides novel evidences demonstrating the presence of miR-31-mediated post-transcriptional regulation of SIRT3 in OSCC. The disruption of miR-31-SIRT3 cascade and the consequential metabolic aberrances are involved in OSCC progression. •miR-31 targets SIRT3 to drive OSCC invasion.•OSCC tumors exhibit miR-31 upregulation and SIRT3 downregulation.•miR-31-SIRT3 disruption underlies the mitochondrial disturbance.•miR-31-SIRT3 cascade induces ROS, glycolytic metabolism and lactate production.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2019.04.028