Utilization of the allen gene expression atlas to gain further insight into glucocorticoid physiology in the adult mouse brain

The neuroanatomic variation of the genomic expression of 25 genes involved in the regulation of glucocorticoid dynamics has been highlighted across 96 regions of the adult mouse brain. These genes (i) characterise the sensitivity of the brain cells to glucocorticoid stimulation, (ii) characterise the glucocorticoid-synthesizing capacity of these cells, (iii) lead to attenuated glucocorticoid stimulation (due to its deactivation or excretion or transfer of precursors to other biosynthetic pathways), and finally (iv) express other stimuli that synergize with the hormonal stimulation. [Display omitted] •GC-sensitive cortical regions relate to saliency processing and executive function.•GC-sensitive subcortical regions are thalami, lateral amygdala and median eminence.•The mouse brain can enzymatically support local GC biosynthesis.•GC and BDNF molecular pathways are highly correlated.•Hippocampus shows high plasticity in responding to and regulating GC stimulation. Glucocorticoid neurodynamics are the most crucial determinant of the hormonal effects in the mammalian brain, and depend on multiple parallel receptor and enzymatic systems, responsible for effectively binding with the hormone (and mediating its downstream molecular effects) and altering the local glucocorticoid content (by adding, removing or degrading glucocorticoids), respectively. In this study, we combined different computational tools to extract, process and visualize the gene expression data of 25 genes across 96 regions of the adult C57Bl/6J mouse brain, implicated in glucocorticoid neurodynamics. These data derive from the anatomic gene expression atlas of the adult mouse brain of the Allen Institute for Brain Science, captured via the in situ hybridization technique. A careful interrogation of the datasets referring to these 25 genes of interest, based on a targeted, prior knowledge-driven approach, revealed useful pieces of information on spatial differences in the glucocorticoid-sensitive receptors, in the regional capacity for local glucocorticoid biosynthesis, excretion, conversion to other biologically active forms and degradation. These data support the importance of the corticolimbic system of the mammalian brain in mediating glucocorticoid effects, and particularly hippocampus, as well as the need for intensifying the research efforts on the hormonal role in sensory processing, executive control function, its interplay with brain-derived neurotrophic factor and the molecular basis