H1N1 hemagglutinin-specific HLA-DQ6-restricted CD4+ T cells can be readily detected in narcolepsy type 1 patients and healthy controls

Following the 2009 H1N1 influenza pandemic, an increased risk of narcolepsy type 1 was observed. Homology between an H1N1 hemagglutinin and two hypocretin sequences has been reported. T cell reactivity to these peptides was assessed in 81 narcolepsy type 1 patients and 19 HLA-DQ6-matched healthy controls. HLA-DQ6-restricted H1N1 hemagglutinin-specific T cell responses were detected in 28.4% of patients and 15.8% of controls. Despite structural homology between HLA-DQ6-hypocretin and -H1N1 peptide complexes, T cell cross-reactivity was not detected. These results indicate that it is unlikely that cross-reactivity between H1N1 hemagglutinin and hypocretin peptides presented by HLA-DQ6 is involved in the development of narcolepsy. [Display omitted] •Hypocretin and H1N1-hemagglutinin peptides bound to HLA-DQ6 show structural homology.•H1N1-hemagglutinin-specific CD4+ T cells detected in narcolepsy type 1 patients and controls.•Cross-reactivity between H1N1-hemagglutinin and hypocretin peptides not observed.•H1N1-hemagglutinin-specific CD4+ T cell reactivity is HLA-DQ6-restricted.•No biased expression in H1N1-hemagglutinin-specific T cell receptor repertoire.