Determination of aflatoxins in foods using HPLC and a commercial ELISA system

A commercially available enzyme linked immunosorbent assay (ELISA) system and a high performance liquid chromatography (HPLC) method were used simultaneously to analyze 178 samples of foodstuffs for total aflatoxins. High correlation coefficient values between results of the two methodologies were o...

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Veröffentlicht in:	Journal of food protection 1991-04, Vol.54 (4), p.291-294
Hauptverfasser:	Azer, M. (Division of Analytical Laboratories, New South Wales Australia), Cooper, C
Format:	Artikel
Sprache:	eng
Schlagworte:	AFLATOXINAS AFLATOXINE AFLATOXINS ALIMENTOS ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE Biological and medical sciences CHROMATOGRAPHIE CHROMATOGRAPHY CONTAMINACION CONTAMINATION CROMATOGRAFIA ELISA Food industries Food microbiology FOODS Fundamental and applied biological sciences. Psychology LIQUID CHROMATOGRAPHY PRODUIT ALIMENTAIRE QUANTITATIVE ANALYSIS QUANTITATIVE TECHNIQUES TEST ELISA
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Beschreibung
Zusammenfassung:	A commercially available enzyme linked immunosorbent assay (ELISA) system and a high performance liquid chromatography (HPLC) method were used simultaneously to analyze 178 samples of foodstuffs for total aflatoxins. High correlation coefficient values between results of the two methodologies were obtained (>0.96) with nuts, nut products, peanuts, and peanut butter. However, poor correlation was obtained from results of cereals and grain samples. The ELISA system demonstrated a high degree of reproducibility between wells (p
ISSN:	0362-028X 1944-9097
DOI:	10.4315/0362-028x-54.4.291