Realization of 19F MRI oximetry method using perfluorodecalin

Objective To identify the technical aspects of the potential use of clinically approved perfluorodecalin (PFD, C 10 F 18 ) for 19 F magnetic resonance imaging (MRI) oximetry method at high magnetic field 7.05 T. Materials and methods 19 F T 1 measurements were made on a set of PFD samples with different oxygen contents (0%, 21%, and 100%) at room (21 °C) and body temperature (37 °C). In vivo MRI studies were carried out on one healthy rat and two rats with C6 brain glioma. Results The selective excitation of the magnetically equivalent 19 F nuclei of CF 2 groups of trans -isomer of PFD, which give a doublet at a frequency of about − 140 ppm (in relation the chemical shift of trifluoroacetic acid, which is − 76.55 ppm) should be done for correct implementation of 19 F MRI oximetry method. The amount of PFD equal to 30 μl is the optimal for obtaining reliable data on the measured T 1 values. In this case, the standard deviation of T 1 does not exceed 5%. In vivo MRI studies showed that the values of the partial pressure of oxygen (pO 2 ) decrease from normal values of about 38 mmHg (healthy brain) to almost 0 mmHg at the last stage of tumor growth. Conclusion The study showed the feasibility of the successful application of PFD for 19 F MRI oximetry method.