Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices

To overcome the effect of other components of complicated biological samples on nanopore stochastic sensing, displacement chemical reaction was utilized to selectively extract the target nucleic acid from whole blood. Given its simplicity and high sensitivity for detecting nucleic acids, our develop...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2018-12, Vol.54 (99), p.13977-13980
Hauptverfasser:	Wang, Liang, Chen, Xiaohan, Zhou, Shuo, Roozbahani, Golbarg M, Zhang, Youwen, Wang, Deqiang, Guan, Xiyun
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological properties Biomarkers - blood Biosensing Techniques - methods blood chemical compounds Chemical reactions Clinical Chemistry Tests - methods Diagnostic software Diagnostic systems Displacement DNA Probes - chemistry DNA, Single-Stranded - chemistry Humans MicroRNAs - blood MicroRNAs - chemistry Nanopores Nucleic Acid Hybridization Nucleic acids Organic chemistry Point-of-Care Systems Porosity Proof of Concept Study Stochastic Processes
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Beschreibung
Zusammenfassung:	To overcome the effect of other components of complicated biological samples on nanopore stochastic sensing, displacement chemical reaction was utilized to selectively extract the target nucleic acid from whole blood. Given its simplicity and high sensitivity for detecting nucleic acids, our developed displacement chemistry-based nanopore sensing strategy offers the potential for fieldable/point-of-care diagnostic applications.
ISSN:	1359-7345 1364-548X 1364-548X
DOI:	10.1039/c8cc07944g