Valorization of egg shell as a detoxifying and buffering agent for efficient polymalic acid production by Aureobasidium pullulans NRRL Y-2311-1 from barley straw hydrolysate

[Display omitted] •Stepwise formulation of a novel medium for polymalic acid production was performed.•Tolerable levels to lignocellulosic inhibitors by A. pullulans were elucidated.•Egg shell was used for detoxification of barley straw hydrolysate for the first time.•Simultaneous detoxification and...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bioresource technology 2019-04, Vol.278, p.130-137
Hauptverfasser: Yegin, Sirma, Saha, Badal C., Kennedy, Gregory J., Leathers, Timothy D.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:[Display omitted] •Stepwise formulation of a novel medium for polymalic acid production was performed.•Tolerable levels to lignocellulosic inhibitors by A. pullulans were elucidated.•Egg shell was used for detoxification of barley straw hydrolysate for the first time.•Simultaneous detoxification and fermentation were carried out.•Maximum polymalic acid production was 43.54 g/L with the yield of 0.48 g/g. Stepwise formulation of a versatile and cost-effective medium based on barley straw hydrolysate and egg shell for efficient polymalic acid production by A. pullulans NRRL Y-2311-1 was carried out for the first time. The strain did not grow and produce polymalic acid when dilute acid pretreated barley straw hydrolysate (total fermentable sugars: 94.60 g/L; furfural: 1.01 g/L; hydroxymethylfurfural: 0.55 g/L; acetic acid: 5.06 g/L) was directly used in medium formulation without detoxification (e.g. charcoal pretreatment). When CaCO3 in the medium formulation was substituted with egg shell powder, efficient production of polymalic acid was achieved without a detoxification step. Utilization of 40 g/L of egg shell powder led to 43.54 g polymalic acid production per L with the productivity of 0.30 g/L/h and yield of 0.48 g/g. The bioprocess strategy used in this study can also be utilized for mass production of several other industrially important microbial organic acids and biomaterials.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2018.12.119