Direct enumeration of coliforms and Escherichia coli by hydrophobic grid membrane filter in 24 hours using MUG

Direct enumeration of coliforms and Escherichia coli by hydrophobic grid membrane filter in 24 hours using MUG

An improved 24-hour grid membrane filter HGMF method for coliform and Escherichia coli enumeration was developed. The new method, which uses a buffered MUG agar for the E. coli portion of the test, was subjected to a pre-collaborative validation study against the 3-tube MPN procedure encompassing 37...

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Veröffentlicht in:Journal of food protection 1990-11, Vol.53 (11), p.948-952
Hauptverfasser: Entis, P. (QA Laboratories, Toronto, Canada), Boleszczuk, P
Format: Artikel
Sprache:eng
Schlagworte:
ALIMENTOS
ANALISIS BIOLOGICO
ANALYSE BIOLOGIQUE
BACTERIA COLIFORME
BACTERIE COLIFORME
Biological and medical sciences
CONTAMINACION
CONTAMINATION
ESCHERICHIA
EXPERIMENTATION EN LABORATOIRE
EXPERIMENTOS EN LABORATORIO
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
METHODE
METODOS
PRODUIT ALIMENTAIRE
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Zusammenfassung:An improved 24-hour grid membrane filter HGMF method for coliform and Escherichia coli enumeration was developed. The new method, which uses a buffered MUG agar for the E. coli portion of the test, was subjected to a pre-collaborative validation study against the 3-tube MPN procedure encompassing 375 naturally contaminated and inoculated samples representing 25 food products. The HGMF/MUG method produced coliform and E. coli counts equivalent to the conventional method. The confirmation rate of MUG-positive colonies in this study was 98.1%
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-53.11.948