Effect of EZH2 knockdown on preimplantation development of porcine parthenogenetic embryos

Effect of EZH2 knockdown on preimplantation development of porcine parthenogenetic embryos

The EZH2 protein endows the polycomb repressive complex 2 (PRC2) with histone lysine methyltransferase activity that is associated with transcriptional repression. Recent investigations have documented crucial roles for EZH2 in mediating X-inactivation, stem cell pluripotency and cancer metastasis....

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Veröffentlicht in:Theriogenology 2019-07, Vol.132, p.95-105
Hauptverfasser: Cai, Qingqing, Niu, Huiran, Zhang, Bingyue, Shi, Xuan, Liao, Mengqin, Chen, Zihao, Mo, Delin, He, Zuyong, Chen, Yaosheng, Cong, Peiqing
Format: Artikel
Sprache:eng
Schlagworte:
Equilibrium
EZH2
H3K27me3
Porcine
Preimplantation development
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Zusammenfassung:The EZH2 protein endows the polycomb repressive complex 2 (PRC2) with histone lysine methyltransferase activity that is associated with transcriptional repression. Recent investigations have documented crucial roles for EZH2 in mediating X-inactivation, stem cell pluripotency and cancer metastasis. However, there is little evidence demonstrating the maternal effect of EZH2 on porcine preimplantation development. Here, we took parthenogenetic activation embryos to eliminate the confounding paternal influence. We showed that the dynamic expression of EZH2 during early development was accompanied by changes in H3K27me3 levels. Depletion of EZH2 in MII oocytes by small interfering RNA not only impaired embryonic development at the blastocyst stage (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.04.007