Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria
The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants. A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment. This synbiotic relationship is facilitated...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2019-06, Vol.91 (11), p.7254-7265 |
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| creator | Amicucci, Matthew J Galermo, Ace G Guerrero, Andres Treves, Guy Nandita, Eshani Kailemia, Muchena J Higdon, Shawn M Pozzo, Tania Labavitch, John M Bennett, Alan B Lebrilla, Carlito B |
| description | The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants. A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment. This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown. In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation. The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches. This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community. The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future. |
| doi_str_mv | 10.1021/acs.analchem.9b00789 |
| format | Article |
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A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment. This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown. In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation. The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches. This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community. The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.9b00789</identifier><identifier>PMID: 30983332</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Analytical methods ; Bacteria ; Carbohydrates ; Chemistry ; Communities ; Consortia ; Corn ; Depolymerization ; Galactose ; Microorganisms ; Monosaccharides ; Mucilage ; Oligosaccharides ; Organic chemistry ; Polysaccharides ; Quantitation ; Saccharides</subject><ispartof>Analytical chemistry (Washington), 2019-06, Vol.91 (11), p.7254-7265</ispartof><rights>Copyright American Chemical Society Jun 4, 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a473t-77f1ebce352a2022dab91702cd2796e3413f71460a38fe9f0a2c674223350b8a3</citedby><cites>FETCH-LOGICAL-a473t-77f1ebce352a2022dab91702cd2796e3413f71460a38fe9f0a2c674223350b8a3</cites><orcidid>0000-0001-7190-5323 ; 0000-0002-5662-1987 ; 0000-0002-1392-9252 ; 0000-0001-9359-1038</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.9b00789$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.9b00789$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30983332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amicucci, Matthew J</creatorcontrib><creatorcontrib>Galermo, Ace G</creatorcontrib><creatorcontrib>Guerrero, Andres</creatorcontrib><creatorcontrib>Treves, Guy</creatorcontrib><creatorcontrib>Nandita, Eshani</creatorcontrib><creatorcontrib>Kailemia, Muchena J</creatorcontrib><creatorcontrib>Higdon, Shawn M</creatorcontrib><creatorcontrib>Pozzo, Tania</creatorcontrib><creatorcontrib>Labavitch, John M</creatorcontrib><creatorcontrib>Bennett, Alan B</creatorcontrib><creatorcontrib>Lebrilla, Carlito B</creatorcontrib><title>Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. 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This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community. The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.</description><subject>Analytical chemistry</subject><subject>Analytical methods</subject><subject>Bacteria</subject><subject>Carbohydrates</subject><subject>Chemistry</subject><subject>Communities</subject><subject>Consortia</subject><subject>Corn</subject><subject>Depolymerization</subject><subject>Galactose</subject><subject>Microorganisms</subject><subject>Monosaccharides</subject><subject>Mucilage</subject><subject>Oligosaccharides</subject><subject>Organic chemistry</subject><subject>Polysaccharides</subject><subject>Quantitation</subject><subject>Saccharides</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kUFP2zAUx61paBTYN5gmS7vskvL8nMTJboMBRSoCie0cvTgONUrrznYO7afHVQsHDpws27___0nvx9g3AVMBKM5JhymtaNALs5zWLYCq6k9sIgqErKwq_MwmACAzVADH7CSEZwAhQJRf2LGEupJS4oRtH6OnaJ42vHeep8uo4-hp4FfDqG1H0boVdz1_cMMmkNYL8rYz4df7f-J3ZLeG36XXgZ4MjwuKfEa-dT7wP5a2Lnq3XljNL0hH4y2dsaOehmC-Hs5T9u_66u_lLJvf39xe_p5nlCsZM6V6YVptZIGEgNhRWwsFqDtUdWlkLmSvRF4Cyao3dQ-EulQ5opQFtBXJU_Zz37v27v9oQmyWNmgzDLQybgwNokiLylUBCf3xDn12o09L3lF5iUUBVZmofE9p70Lwpm_W3i7JbxoBzc5Nk9w0r26ag5sU-34oH9ul6d5CrzISAHtgF38b_GHnCxHZnks</recordid><startdate>20190604</startdate><enddate>20190604</enddate><creator>Amicucci, Matthew J</creator><creator>Galermo, Ace G</creator><creator>Guerrero, Andres</creator><creator>Treves, Guy</creator><creator>Nandita, Eshani</creator><creator>Kailemia, Muchena J</creator><creator>Higdon, Shawn M</creator><creator>Pozzo, Tania</creator><creator>Labavitch, John M</creator><creator>Bennett, Alan B</creator><creator>Lebrilla, Carlito B</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7190-5323</orcidid><orcidid>https://orcid.org/0000-0002-5662-1987</orcidid><orcidid>https://orcid.org/0000-0002-1392-9252</orcidid><orcidid>https://orcid.org/0000-0001-9359-1038</orcidid></search><sort><creationdate>20190604</creationdate><title>Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria</title><author>Amicucci, Matthew J ; Galermo, Ace G ; Guerrero, Andres ; Treves, Guy ; Nandita, Eshani ; Kailemia, Muchena J ; Higdon, Shawn M ; Pozzo, Tania ; Labavitch, John M ; Bennett, Alan B ; Lebrilla, Carlito B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a473t-77f1ebce352a2022dab91702cd2796e3413f71460a38fe9f0a2c674223350b8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical chemistry</topic><topic>Analytical methods</topic><topic>Bacteria</topic><topic>Carbohydrates</topic><topic>Chemistry</topic><topic>Communities</topic><topic>Consortia</topic><topic>Corn</topic><topic>Depolymerization</topic><topic>Galactose</topic><topic>Microorganisms</topic><topic>Monosaccharides</topic><topic>Mucilage</topic><topic>Oligosaccharides</topic><topic>Organic chemistry</topic><topic>Polysaccharides</topic><topic>Quantitation</topic><topic>Saccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amicucci, Matthew J</creatorcontrib><creatorcontrib>Galermo, Ace G</creatorcontrib><creatorcontrib>Guerrero, Andres</creatorcontrib><creatorcontrib>Treves, Guy</creatorcontrib><creatorcontrib>Nandita, Eshani</creatorcontrib><creatorcontrib>Kailemia, Muchena J</creatorcontrib><creatorcontrib>Higdon, Shawn M</creatorcontrib><creatorcontrib>Pozzo, Tania</creatorcontrib><creatorcontrib>Labavitch, John M</creatorcontrib><creatorcontrib>Bennett, Alan B</creatorcontrib><creatorcontrib>Lebrilla, Carlito B</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amicucci, Matthew J</au><au>Galermo, Ace G</au><au>Guerrero, Andres</au><au>Treves, Guy</au><au>Nandita, Eshani</au><au>Kailemia, Muchena J</au><au>Higdon, Shawn M</au><au>Pozzo, Tania</au><au>Labavitch, John M</au><au>Bennett, Alan B</au><au>Lebrilla, Carlito B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2019-06-04</date><risdate>2019</risdate><volume>91</volume><issue>11</issue><spage>7254</spage><epage>7265</epage><pages>7254-7265</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants. A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment. This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown. In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation. The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches. This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community. The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>30983332</pmid><doi>10.1021/acs.analchem.9b00789</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-7190-5323</orcidid><orcidid>https://orcid.org/0000-0002-5662-1987</orcidid><orcidid>https://orcid.org/0000-0002-1392-9252</orcidid><orcidid>https://orcid.org/0000-0001-9359-1038</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical chemistry Analytical methods Bacteria Carbohydrates Chemistry Communities Consortia Corn Depolymerization Galactose Microorganisms Monosaccharides Mucilage Oligosaccharides Organic chemistry Polysaccharides Quantitation Saccharides |
| title | Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria |
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