Abscisic acid-generating bacteria can reduce Cd concentration in pakchoi grown in Cd-contaminated soil

Contamination of vegetable plants with cadmium (Cd) has become a serious issue in recent years. In the present study, pakchoi (Brassica chinensis L.) grown in Cd-contaminated soil inoculated with abscisic acid (ABA)-generating bacteria, Azospirillum brasilense and Bacillus subtilis, showed 28%–281% and 26%–255% greater biomass, and 40%–79% and 43%–77% lower Cd concentrations, respectively, than those of the controlbacteria-free plants. These treatments also alleviated the Cd-induced photosynthesis inhibition and oxidative damage (indicated by malondialdehyde [MDA], H2O2, and O2• −). Furthermore, the application of bacteria also remarkably improved the levels of antioxidant-related compounds (total phenolics, total flavonoids, ascorbate, and 2,2-diphenyl-1-picrylhydrazyl [DPPH] activity) and nutritional quality (soluble sugar and soluble protein) in the Cd-supplied plants. Based on these results, we conclude that the application of ABA-generating bacteria might be an alternative strategy for improving the biomass production and quality of vegetable plants grown in Cd-contaminated soil. [Display omitted] •B. subtilis and A. brasilense application yielded a greater biomass and decreased Cd levels in pakchoi.•Cd-induced photosynthesis inhibition and oxidative damages were restored by bacterial inoculation.•Levels of antioxidative compounds were elevated by bacterial inoculation.•The nutritional quality of the edible parts was improved by bacterial inoculation.